Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 123691..124334 Replicon plasmid unnamed1
Accession NZ_CP055001
Organism Shigella flexneri strain STIN_80

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HUZ55_RS24270 Protein ID WP_001044768.1
Coordinates 123918..124334 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HUZ55_RS24265 Protein ID WP_001261287.1
Coordinates 123691..123921 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ55_RS24245 (118871) 118871..120004 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
HUZ55_RS24250 (120267) 120267..121904 - 1638 WP_223201785.1 hypothetical protein -
HUZ55_RS24255 (121955) 121955..122785 - 831 WP_223201786.1 hypothetical protein -
HUZ55_RS24260 (122837) 122837..123385 - 549 WP_039002808.1 hypothetical protein -
HUZ55_RS24265 (123691) 123691..123921 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ55_RS24270 (123918) 123918..124334 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ55_RS24275 (124496) 124496..126634 - 2139 WP_039002805.1 AAA family ATPase -
HUZ55_RS24280 (127099) 127099..128094 + 996 WP_000246636.1 hypothetical protein -
HUZ55_RS24285 (128137) 128137..129030 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3')-Ia / sul3 / blaTEM-1B / lnu(F) / aac(3)-IId - 1..132056 132056
- flank IS/Tn - - 129167..129670 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T160913 WP_001044768.1 NZ_CP055001:123918-124334 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T160913 NZ_CP070963:95350-95766 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT160913 WP_001261287.1 NZ_CP055001:123691-123921 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT160913 NZ_CP070963:95123-95353 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References