Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3933228..3933847 Replicon chromosome
Accession NZ_CP054997
Organism Klebsiella pneumoniae strain STIN_84

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ56_RS19075 Protein ID WP_002892050.1
Coordinates 3933629..3933847 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ56_RS19070 Protein ID WP_002892066.1
Coordinates 3933228..3933602 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ56_RS19060 (HUZ56_19010) 3928380..3929573 + 1194 WP_086519390.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ56_RS19065 (HUZ56_19015) 3929596..3932742 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ56_RS19070 (HUZ56_19020) 3933228..3933602 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ56_RS19075 (HUZ56_19025) 3933629..3933847 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ56_RS19080 (HUZ56_19030) 3934006..3934572 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ56_RS19085 (HUZ56_19035) 3934544..3934684 - 141 WP_004147370.1 hypothetical protein -
HUZ56_RS19090 (HUZ56_19040) 3934705..3935175 + 471 WP_002892026.1 YlaC family protein -
HUZ56_RS19095 (HUZ56_19045) 3935150..3936601 - 1452 WP_211603584.1 PLP-dependent aminotransferase family protein -
HUZ56_RS19100 (HUZ56_19050) 3936702..3937400 + 699 WP_002892021.1 GNAT family protein -
HUZ56_RS19105 (HUZ56_19055) 3937397..3937537 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ56_RS19110 (HUZ56_19060) 3937537..3937800 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160871 WP_002892050.1 NZ_CP054997:3933629-3933847 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T160871 NZ_CP070959:c1224960-1224694 [Thiomicrospira microaerophila]
TTGGCCTGGACAATTGAATATACTGACACAAGCCTTAAGTCACTTAAAAAGATTGATAAAACTTGGCAAAAACGCATCCT
CGACTATTTAGATAATACCGTCGCAGTGCGAGATAATCCACGCGATTATGGCAAGCCATTAGTTGGCCATTTAAGTGGTT
TGTGGCGTTATCGTGTGGGTAATTATCGAGTTATCTGTCAAATCCAAGATCAAGATTTAATTATCTTAACCACCCAGATT
AGCCATCGAAAAGATCTGTATCAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT160871 WP_002892066.1 NZ_CP054997:3933228-3933602 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT160871 NZ_CP070959:c1225168-1224944 [Thiomicrospira microaerophila]
ATGTTAGCCGTGCGCTTGGATGCTGAAATGGAGCAGGAGTTAGATAGGTTGGCGAGAGAGACAGGACGTACTAAAACGTT
TTATGTCAAAGAAGCTTTAGCACACTATTTAGCCGATATTAAAGATTATTATGATGCTCAAGATGTGCTTAACAATGTTA
AGCTTGGAAAAGAAAAAGTTCATAGTTCAGCCGATGTGAGAAATACGCTTGGCCTGGACAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References