Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4058029..4058605 Replicon chromosome
Accession NZ_CP054992
Organism Shigella flexneri strain STIN_87

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HUZ57_RS19725 Protein ID WP_001297643.1
Coordinates 4058318..4058605 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HUZ57_RS19720 Protein ID WP_000063156.1
Coordinates 4058029..4058331 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ57_RS19705 (4054625) 4054625..4056775 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HUZ57_RS19710 (4056825) 4056825..4057028 + 204 WP_000467859.1 YbdD/YjiX family protein -
HUZ57_RS19715 (4057039) 4057039..4057995 + 957 WP_001297640.1 GTPase -
HUZ57_RS19720 (4058029) 4058029..4058331 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HUZ57_RS19725 (4058318) 4058318..4058605 - 288 WP_001297643.1 BrnT family toxin Toxin
HUZ57_RS19730 (4058804) 4058804..4059501 - 698 Protein_3876 IS1 family transposase -
HUZ57_RS19735 (4059558) 4059558..4059653 + 96 Protein_3877 dienelactone hydrolase family protein -
HUZ57_RS19740 (4059799) 4059799..4061031 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
HUZ57_RS19745 (4061072) 4061072..4062352 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T160855 WP_001297643.1 NZ_CP054992:c4058605-4058318 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T160855 NZ_CP070954:c2595127-2595024 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT160855 WP_000063156.1 NZ_CP054992:c4058331-4058029 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT160855 NZ_CP070954:2594986-2595253 [Escherichia fergusonii]
AAAGTCAGCGAAGGGAATTTTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCGT
TCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References