Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3455161..3455779 Replicon chromosome
Accession NZ_CP054992
Organism Shigella flexneri strain STIN_87

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ57_RS16800 Protein ID WP_001291435.1
Coordinates 3455561..3455779 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ57_RS16795 Protein ID WP_000344800.1
Coordinates 3455161..3455535 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ57_RS16785 (3450250) 3450250..3451443 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ57_RS16790 (3451466) 3451466..3454615 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ57_RS16795 (3455161) 3455161..3455535 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ57_RS16800 (3455561) 3455561..3455779 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ57_RS16805 (3455951) 3455951..3456502 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ57_RS16810 (3456618) 3456618..3457088 + 471 WP_000136192.1 YlaC family protein -
HUZ57_RS16815 (3457252) 3457252..3458802 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ57_RS16820 (3458844) 3458844..3459197 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ57_RS16830 (3459576) 3459576..3459887 + 312 WP_000409911.1 MGMT family protein -
HUZ57_RS16835 (3459918) 3459918..3460490 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T160852 WP_001291435.1 NZ_CP054992:3455561-3455779 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T160852 NZ_CP070954:1769824-1770075 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT160852 WP_000344800.1 NZ_CP054992:3455161-3455535 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT160852 NZ_CP070954:1770072-1770326 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACGGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References