Detailed information of TA system TA160828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 54795..55438
• Replicon: plasmid unnamed1
• Accession: NZ_CP054985
• Organism: Klebsiella pneumoniae strain STIN_90

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HUZ59_RS26105
• Protein ID: WP_239619444.1
• Coordinates: 55022..55438 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HUZ59_RS26100
• Protein ID: WP_001261282.1
• Coordinates: 54795..55025 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ59_RS26065 (HUZ59_26015)	49804..50979	+	1176	Protein_48	IS3 family transposase	-
HUZ59_RS26070 (HUZ59_26020)	51026..51241	+	216	WP_129586912.1	hypothetical protein	-
HUZ59_RS26075 (HUZ59_26025)	51361..52146	-	786	WP_050485696.1	site-specific integrase	-
HUZ59_RS26080 (HUZ59_26030)	52192..52713	-	522	WP_032448294.1	hypothetical protein	-
HUZ59_RS26085 (HUZ59_26035)	52771..53163	-	393	WP_074422814.1	hypothetical protein	-
HUZ59_RS26090 (HUZ59_26040)	53272..54216	-	945	WP_032448293.1	hypothetical protein	-
HUZ59_RS26095 (HUZ59_26045)	54416..54838	-	423	WP_239619443.1	hypothetical protein	-
HUZ59_RS26100 (HUZ59_26050)	54795..55025	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ59_RS26105 (HUZ59_26055)	55022..55438	+	417	WP_239619444.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ59_RS26110 (HUZ59_26060)	55512..57074	+	1563	WP_004206609.1	AAA family ATPase	-
HUZ59_RS26115 (HUZ59_26065)	57059..58081	+	1023	WP_032439672.1	helicase UvrD	-
HUZ59_RS26120 (HUZ59_26070)	58626..59534	+	909	WP_032448316.1	HNH endonuclease	-
HUZ59_RS26125 (HUZ59_26075)	59720..60070	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..221140	221140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15109.58 Da
• Isoelectric Point: 7.1084

>T160828 WP_239619444.1 NZ_CP054985:55022-55438 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAMAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T160828 NZ_CP070951:509490-509780 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT160828 WP_001261282.1 NZ_CP054985:54795-55025 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT160828 NZ_CP070951:509258-509500 [Escherichia fergusonii]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGAAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3