160748

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5135130..5135755 Replicon chromosome
Accession NZ_CP054967
Organism Klebsiella pneumoniae strain STIN_94

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ62_RS24935 Protein ID WP_002882817.1
Coordinates 5135130..5135513 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ62_RS24940 Protein ID WP_004150355.1
Coordinates 5135513..5135755 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ62_RS24920 (5132496) 5132496..5133398 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ62_RS24925 (5133395) 5133395..5134030 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ62_RS24930 (5134027) 5134027..5134956 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ62_RS24935 (5135130) 5135130..5135513 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ62_RS24940 (5135513) 5135513..5135755 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ62_RS24945 (5135960) 5135960..5136877 + 918 WP_021466676.1 alpha/beta hydrolase -
HUZ62_RS24950 (5136891) 5136891..5137832 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ62_RS24955 (5137877) 5137877..5138314 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ62_RS24960 (5138311) 5138311..5139171 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ62_RS24965 (5139165) 5139165..5139764 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T160748 WP_002882817.1 NZ_CP054967:c5135513-5135130 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T160748 NZ_CP070906:c1861526-1861152 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT160748 WP_004150355.1 NZ_CP054967:c5135755-5135513 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT160748 NZ_CP070906:c1861983-1861615 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References