Detailed information of TA system TA160676

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4553011..4553846
• Replicon: chromosome
• Accession: NZ_CP054940
• Organism: Escherichia coli strain MS6192

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q707F1
• Locus tag: MS6192_RS21940
• Protein ID: WP_001094445.1
• Coordinates: 4553011..4553388 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MS6192_RS21945
• Protein ID: WP_024174331.1
• Coordinates: 4553478..4553846 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6192_RS21920	4549103..4550641	-	1539	WP_001187170.1	lysine decarboxylation/transport transcriptional activator CadC	-
MS6192_RS21925	4551390..4552232	-	843	WP_001280499.1	DUF4942 domain-containing protein	-
MS6192_RS21930	4552317..4552514	-	198	WP_000838536.1	DUF957 domain-containing protein	-
MS6192_RS21935	4552526..4553014	-	489	WP_000761717.1	hypothetical protein	-
MS6192_RS21940	4553011..4553388	-	378	WP_001094445.1	TA system toxin CbtA family protein	Toxin
MS6192_RS21945	4553478..4553846	-	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MS6192_RS21950	4553920..4554141	-	222	WP_000692346.1	DUF987 domain-containing protein	-
MS6192_RS21955	4554210..4554686	-	477	WP_001186725.1	RadC family protein	-
MS6192_RS21960	4554702..4555187	-	486	WP_000214415.1	antirestriction protein	-
MS6192_RS21965	4555279..4555611	-	333	Protein_4276	DUF932 domain-containing protein	-
MS6192_RS21970	4555614..4556394	-	781	Protein_4277	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4541614..4572368	30754
	-	flank	IS/Tn	-	-	4556116..4556394	278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14194.10 Da
• Isoelectric Point: 6.8518

>T160676 WP_001094445.1 NZ_CP054940:c4553388-4553011 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T160676 NZ_CP070873:c295246-294839 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT160676 WP_024174331.1 NZ_CP054940:c4553846-4553478 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT160676 NZ_CP070873:c295493-295227 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A454A7U6