Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3213477..3214131 Replicon chromosome
Accession NZ_CP054940
Organism Escherichia coli strain MS6192

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag MS6192_RS15630 Protein ID WP_000244772.1
Coordinates 3213477..3213884 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MS6192_RS15635 Protein ID WP_000354046.1
Coordinates 3213865..3214131 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6192_RS15610 3209434..3211167 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
MS6192_RS15615 3211173..3211883 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MS6192_RS15620 3211908..3212804 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MS6192_RS15625 3212916..3213437 + 522 WP_001055874.1 flavodoxin FldB -
MS6192_RS15630 3213477..3213884 - 408 WP_000244772.1 protein YgfX Toxin
MS6192_RS15635 3213865..3214131 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MS6192_RS15640 3214374..3215354 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MS6192_RS15645 3215431..3216090 - 660 WP_000250274.1 hemolysin III family protein -
MS6192_RS15650 3216254..3216565 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MS6192_RS15655 3216610..3218043 + 1434 WP_024198262.1 6-phospho-beta-glucosidase BglA -
MS6192_RS15660 3218100..3218843 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T160671 WP_000244772.1 NZ_CP054940:c3213884-3213477 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T160671 NZ_CP070865:c308809-308510 [Shewanella putrefaciens]
ATGAAAAATAAACGCTATAAACTGAGTAAGTTAGCTCAAACTCACTTACTAAAGATTAAAGACTACACCATTCAACACTT
TTCTGAGTCGCAGTGGCACAAATACAAAGAAAGCCTAATATCCGGCTTGCAAATGTTAGCCGATAACCCCGGATTAGGCA
GAAGCTGCGAAGATATTTACCCCAACGGATTCTACTTTCCGATTGCTAAACACACCGCCTACTTCACAAAAGAAGACGAC
TTCATCCTCATCGTTGCCATGTTAGGCCAGCCACAACTACCACAAAATCATCTGAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT160671 WP_000354046.1 NZ_CP054940:c3214131-3213865 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT160671 NZ_CP070865:c309060-308818 [Shewanella putrefaciens]
GTGCAAACATTAACAGCCAATGACGCCAAACGAAATTTCGGTGAGTTACTGCTAAACGCTCAACGTGAACCCATCAAGAT
CAGCAAAAACAGTAAAGATGCGGTTGTTGTGATGTCTATCCACGATTACGAACAGCTTGAGGCGATGAAAGCCGACTATC
TGAAGCACTGTTTCAACGCAGCCAAAGAAGACTTAGCGAAAGGTAATATTGTTGATGGTGAAGATTTTCTTAACGCCCTG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References