Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 318509..319122 Replicon chromosome
Accession NZ_CP054867
Organism Pseudomonas chlororaphis strain PCLRT02

Toxin (Protein)


Gene name hicA Uniprot ID A0A285EYZ4
Locus tag HUT27_RS01390 Protein ID WP_009041685.1
Coordinates 318509..318691 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A554PT90
Locus tag HUT27_RS01395 Protein ID WP_009046450.1
Coordinates 318721..319122 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUT27_RS01365 (HUT27_01365) 313692..315656 + 1965 WP_164485207.1 choline transporter BetT -
HUT27_RS01370 (HUT27_01370) 315769..316026 - 258 WP_009046444.1 hypothetical protein -
HUT27_RS01375 (HUT27_01375) 316079..316567 - 489 WP_124354496.1 DoxX family protein -
HUT27_RS01380 (HUT27_01380) 316624..317364 - 741 WP_016702555.1 SDR family oxidoreductase -
HUT27_RS01385 (HUT27_01385) 317494..318384 + 891 WP_041985374.1 LysR family transcriptional regulator -
HUT27_RS01390 (HUT27_01390) 318509..318691 + 183 WP_009041685.1 type II toxin-antitoxin system HicA family toxin Toxin
HUT27_RS01395 (HUT27_01395) 318721..319122 + 402 WP_009046450.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUT27_RS01400 (HUT27_01400) 319692..320729 + 1038 WP_016702559.1 L-glyceraldehyde 3-phosphate reductase -
HUT27_RS01405 (HUT27_01405) 320860..321288 - 429 WP_023970008.1 type II toxin-antitoxin system VapC family toxin -
HUT27_RS01410 (HUT27_01410) 321285..321530 - 246 WP_016702561.1 plasmid stabilization protein -
HUT27_RS01415 (HUT27_01415) 321670..322509 - 840 WP_124354497.1 taurine dioxygenase -
HUT27_RS01420 (HUT27_01420) 322745..323584 - 840 WP_009046455.1 taurine ABC transporter permease TauC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6933.08 Da        Isoelectric Point: 10.4588

>T160540 WP_009041685.1 NZ_CP054867:318509-318691 [Pseudomonas chlororaphis]
MRSRELIRMIEEDGWYLIAVKGSHHQYKHLHKPGRVTIPHPDADLPRGTIHSILKQAGLK

Download         Length: 183 bp

>T160540 NZ_CP070564:c4823141-4822773 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14596.44 Da        Isoelectric Point: 4.9133

>AT160540 WP_009046450.1 NZ_CP054867:318721-319122 [Pseudomonas chlororaphis]
MKFPVVLHKDADSGYGVIVPDVPGCFSAGHTAAQAFENVKEALSLHYEGLVADGEPLPQVHEVDVHIDNPDYAGGVWGVI
EFDITPYFGKAVRFNATLPEQLLERIDQTVKRDQRYRSRSGFLAAAALRELSA

Download         Length: 402 bp

>AT160540 NZ_CP070564:c4823359-4823138 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285EYZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A554PT90

References