Detailed information of TA system TA160464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5503175..5503761
• Replicon: chromosome
• Accession: NZ_CP054791
• Organism: Pseudomonas aeruginosa strain SE5430

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUK74_RS26035
• Protein ID: WP_003120987.1
• Coordinates: 5503462..5503761 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUK74_RS26030
• Protein ID: WP_003448662.1
• Coordinates: 5503175..5503465 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUK74_RS26015 (HUK74_25990)	5498757..5500646	+	1890	WP_179063445.1	hypothetical protein	-
HUK74_RS26020 (HUK74_25995)	5500643..5502619	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HUK74_RS26025 (HUK74_26000)	5502760..5503104	+	345	WP_016851612.1	hypothetical protein	-
HUK74_RS26030 (HUK74_26005)	5503175..5503465	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUK74_RS26035 (HUK74_26010)	5503462..5503761	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUK74_RS26040 (HUK74_26015)	5503963..5505087	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HUK74_RS26045 (HUK74_26020)	5505087..5506796	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
HUK74_RS26050 (HUK74_26025)	5506800..5508125	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HUK74_RS26055 (HUK74_26030)	5508115..5508648	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5469648..5556086	86438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T160464 WP_003120987.1 NZ_CP054791:c5503761-5503462 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T160464 NZ_CP070508:4631866-4632177 [Serratia ureilytica]
ATGCATATCATTTCCGGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGGTGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAGTACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCGTCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT160464 WP_003448662.1 NZ_CP054791:c5503465-5503175 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT160464 NZ_CP070508:4632174-4632596 [Serratia ureilytica]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTACCCTTATTGCAGGGCAGCCACGCTCAGCAAGA
CTACCGAGAAGCGCTCGAATTGGTGGAATATCTGCTGGAAAACGATGAACGCCATCCGCTGATTGACATGTTGGCAAAAA
AAATCGCCGAATATGAAGACAACAGCGCCGAATTTACTGCATTCAACCAGCGTATCGCCCAACTCCCGCAGGGGGTGGCC
GCGCTTCGCGTATTGATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGCAAAAAGTCGTTGGTCAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCAGCCC
TGTTCCTGCCGGAAGCGGATTAA

Structures

Toxin

• AlphaFold DB: G8CP73