Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 132676..132929 Replicon plasmid pKP20194b-p2
Accession NZ_CP054770
Organism Klebsiella pneumoniae strain KP20194b

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag HUS00_RS28750 Protein ID WP_001312851.1
Coordinates 132780..132929 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 132676..132735 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS00_RS28710 128058..128402 - 345 Protein_157 IS6-like element IS26 family transposase -
HUS00_RS28715 128454..129158 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HUS00_RS28720 129222..129383 + 162 Protein_159 DNA helicase -
HUS00_RS28725 129403..130149 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
HUS00_RS28730 130204..130764 + 561 WP_000139328.1 fertility inhibition protein FinO -
HUS00_RS28735 130896..131096 + 201 WP_015059022.1 hypothetical protein -
HUS00_RS28740 131482..132081 + 600 WP_032083981.1 hypothetical protein -
HUS00_RS28745 132245..132475 + 231 WP_001736714.1 hypothetical protein -
- 132676..132735 - 60 NuclAT_1 - Antitoxin
- 132676..132735 - 60 NuclAT_1 - Antitoxin
- 132676..132735 - 60 NuclAT_1 - Antitoxin
- 132676..132735 - 60 NuclAT_1 - Antitoxin
HUS00_RS28750 132780..132929 + 150 WP_001312851.1 type I toxin-antitoxin system Hok family toxin Toxin
HUS00_RS28755 133213..133461 + 249 WP_000083837.1 replication regulatory protein RepA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..133772 133772


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-45)


Sequences


Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T160362 WP_001312851.1 NZ_CP054770:132780-132929 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T160362 NZ_CP070471:2220112-2220867 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 60 bp

>AT160362 NZ_CP054770:c132735-132676 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829C8K5


Antitoxin

Download structure file

References