Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4788813..4789623 Replicon chromosome
Accession NZ_CP054750
Organism Klebsiella pneumoniae strain KP20194c3

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HUS03_RS23705 Protein ID WP_002887280.1
Coordinates 4788813..4789346 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUS03_RS23710 Protein ID WP_002887278.1
Coordinates 4789357..4789623 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS03_RS23700 4787644..4788765 + 1122 WP_002887282.1 cupin domain-containing protein -
HUS03_RS23705 4788813..4789346 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
HUS03_RS23710 4789357..4789623 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HUS03_RS23715 4789726..4791159 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
HUS03_RS23720 4791149..4791832 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HUS03_RS23725 4792004..4793389 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
HUS03_RS23730 4793407..4793751 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T160264 WP_002887280.1 NZ_CP054750:c4789346-4788813 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T160264 NZ_CP070421:c104734-104249 [Klebsiella quasipneumoniae]
GTGGGATATGTAACATCACCAGAACCGTTGTCTTCTTTTCATCAGGTGGCGGAGTTTATCAGCGGTGAAGCCGTGCTTGA
TGACTGGTTAAAGCAGAGGAGCCTCAAAAACCAGGCGACTGGAGCGGCCCGAACGTTTGTGGTGTGTAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAATCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATTCCTGTCATTATACTTGCCAGGCTTGCCGTCGATGTATCGTTCCGTGGAAAAGGGCTTGGTGCTGATTT
GCTTCATGACGCAGTGCTTCGTTGCTTTCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCACGCGCTTACGG
AAGAAGCCAAAAACTTCTATATTCATCATGGTTTCAAACCATCACAAAGTCAGCCACGGACATTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT160264 WP_002887278.1 NZ_CP054750:c4789623-4789357 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT160264 NZ_CP070421:c104988-104722 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTTAACCTCAGAGCCAAGGAATCCCAGCGAGCACTCATTGATGCTGCTGCTGAAATCCTTCA
CAAATCACGAACAGATTTCATTCTGGAAATGGCCTGTAAGGCTGCCGAGGATGTCATCCTTGACCGCCGCGTCTTTAATT
TTAACGATGAGCAATACGCCGAGTTCATCGATATGATTGATGCGCCGGTTGCAGATGATCCTGCTATCGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGATATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References