Detailed information of TA system TA160191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2247..2721
• Replicon: plasmid pKP20194d-p5
• Accession: NZ_CP054737
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: HUS06_RS29265
• Protein ID: WP_004197762.1
• Coordinates: 2247..2513 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: HUS06_RS29270
• Protein ID: WP_000879771.1
• Coordinates: 2497..2721 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS29250	811..1190	+	380	Protein_0	hypothetical protein	-
HUS06_RS29255	1216..1761	+	546	WP_023317533.1	hypothetical protein	-
HUS06_RS29260	1827..2177	+	351	WP_004197756.1	hypothetical protein	-
HUS06_RS29265	2247..2513	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS06_RS29270	2497..2721	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
HUS06_RS29275	3388..3600	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
HUS06_RS29280	3607..4116	-	510	WP_032433972.1	MbeB family mobilization protein	-
HUS06_RS29285	4091..4855	-	765	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
HUS06_RS29290	4794..5138	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
HUS06_RS29295	5128..5304	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T160191 WP_004197762.1 NZ_CP054737:c2513-2247 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T160191 NZ_CP070338:2707467-2707907 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT160191 WP_000879771.1 NZ_CP054737:c2721-2497 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT160191 NZ_CP070338:2707193-2707480 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5