Detailed information of TA system TA160183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 119866..120536
• Replicon: plasmid pKP20194d-p1
• Accession: NZ_CP054733
• Organism: Klebsiella pneumoniae strain KP20194d

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: HUS06_RS27525
• Protein ID: WP_004213072.1
• Coordinates: 119866..120309 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: HUS06_RS27530
• Protein ID: WP_004213073.1
• Coordinates: 120306..120536 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS06_RS27490	115277..115552	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
HUS06_RS27495	115615..116106	+	492	WP_011251327.1	DM13 domain-containing protein	-
HUS06_RS27500	116155..117075	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
HUS06_RS27505	117166..117572	+	407	Protein_132	GAF domain-containing protein	-
HUS06_RS27510	118087..118722	-	636	Protein_133	response regulator	-
HUS06_RS27515	119139..119432	+	294	Protein_134	transposase	-
HUS06_RS27520	119436..119852	+	417	Protein_135	restriction endonuclease	-
HUS06_RS27525	119866..120309	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUS06_RS27530	120306..120536	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HUS06_RS27535	121144..122277	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
HUS06_RS27540	122293..122586	+	294	WP_004213076.1	hypothetical protein	-
HUS06_RS27545	122576..122782	-	207	WP_004213077.1	hypothetical protein	-
HUS06_RS27550	123134..123424	+	291	WP_004213078.1	hypothetical protein	-
HUS06_RS27555	123414..124313	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..194896	194896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T160183 WP_004213072.1 NZ_CP054733:c120309-119866 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T160183 NZ_CP070338:2099969-2100379 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT160183 WP_004213073.1 NZ_CP054733:c120536-120306 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT160183 NZ_CP070338:2099743-2099976 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620