Detailed information of TA system TA160131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1500..1974
• Replicon: plasmid pKP20194f-p5
• Accession: NZ_CP054725
• Organism: Klebsiella pneumoniae strain KP20194f

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: HUS08_RS29340
• Protein ID: WP_004197762.1
• Coordinates: 1708..1974 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: HUS08_RS29335
• Protein ID: WP_000879771.1
• Coordinates: 1500..1724 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS08_RS29330	621..833	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
HUS08_RS29335	1500..1724	+	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
HUS08_RS29340	1708..1974	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS08_RS29345	2044..2394	-	351	WP_004197756.1	hypothetical protein	-
HUS08_RS29350	2460..3005	-	546	WP_023317533.1	hypothetical protein	-
HUS08_RS29355	3031..3410	-	380	Protein_6	hypothetical protein	-
HUS08_RS29360	4513..4689	+	177	WP_004197757.1	hypothetical protein	-
HUS08_RS29365	4679..5023	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
HUS08_RS29370	5013..5594	+	582	Protein_9	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T160131 WP_004197762.1 NZ_CP054725:1708-1974 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T160131 NZ_CP070305:c83660-83451 [Staphylococcus sp. SM3655]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT160131 WP_000879771.1 NZ_CP054725:1500-1724 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT160131 NZ_CP070305:c83965-83672 [Staphylococcus sp. SM3655]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5