Detailed information of TA system TA159986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1527513..1528169
• Replicon: chromosome
• Accession: NZ_CP054623
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HUF09_RS07230
• Protein ID: WP_033971890.1
• Coordinates: 1527987..1528169 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUF09_RS07225
• Protein ID: WP_023875730.1
• Coordinates: 1527513..1527941 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF09_RS07185	1523556..1524269	+	714	WP_003159463.1	phage antirepressor KilAC domain-containing protein	-
HUF09_RS07190	1524334..1525095	+	762	WP_198580818.1	helix-turn-helix domain-containing protein	-
HUF09_RS07195	1525092..1525775	+	684	WP_198580819.1	replication P family protein	-
HUF09_RS07200	1525772..1525978	+	207	WP_198580820.1	hypothetical protein	-
HUF09_RS07205	1525975..1526346	+	372	WP_121147581.1	recombinase	-
HUF09_RS07210	1526343..1526690	+	348	WP_058161108.1	RusA family crossover junction endodeoxyribonuclease	-
HUF09_RS07215	1526687..1526986	+	300	WP_073652357.1	hypothetical protein	-
HUF09_RS07220	1526988..1527362	+	375	WP_016852550.1	hypothetical protein	-
HUF09_RS07225	1527513..1527941	-	429	WP_023875730.1	transcriptional regulator	Antitoxin
HUF09_RS07230	1527987..1528169	-	183	WP_033971890.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUF09_RS07235	1528369..1528701	+	333	WP_043494869.1	phage holin, lambda family	-
HUF09_RS07240	1528698..1529315	+	618	WP_128542531.1	glycoside hydrolase family 19 protein	-
HUF09_RS07245	1529312..1529554	+	243	WP_023875728.1	hypothetical protein	-
HUF09_RS07250	1529551..1530021	+	471	WP_124173773.1	lysis system i-spanin subunit Rz	-
HUF09_RS07255	1530018..1530761	+	744	WP_128542530.1	hypothetical protein	-
HUF09_RS07260	1530893..1531420	+	528	WP_058143933.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1504657..1558667	54010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6776.89 Da
• Isoelectric Point: 11.0775

>T159986 WP_033971890.1 NZ_CP054623:c1528169-1527987 [Pseudomonas aeruginosa]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGTKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T159986 NZ_CP070229:4870922-4871173 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15758.92 Da
• Isoelectric Point: 4.6562

>AT159986 WP_023875730.1 NZ_CP054623:c1527941-1527513 [Pseudomonas aeruginosa]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKAHAISEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KIALWNEMVRRDMRKADLCRLLGIAQTQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT159986 NZ_CP070229:4871170-4871424 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAAGGGAAACCAGAACCACTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA