Detailed information of TA system TA159983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 263632..264281
• Replicon: plasmid unnamed5
• Accession: NZ_CP054620
• Organism: Azospirillum oryzae strain KACC 14407

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUE56_RS23425
• Protein ID: WP_149201463.1
• Coordinates: 263632..264030 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUE56_RS23430
• Protein ID: WP_149201464.1
• Coordinates: 264030..264281 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE56_RS23400	258793..259536	-	744	WP_109074740.1	amino acid ABC transporter ATP-binding protein	-
HUE56_RS23405	259619..260428	-	810	WP_149201459.1	transporter substrate-binding domain-containing protein	-
HUE56_RS23410	260464..261117	-	654	WP_149201460.1	amino acid ABC transporter permease	-
HUE56_RS23415	261147..261821	-	675	WP_149201461.1	amino acid ABC transporter permease	-
HUE56_RS23420	262134..263618	+	1485	WP_149201462.1	MFS transporter	-
HUE56_RS23425	263632..264030	-	399	WP_149201463.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUE56_RS23430	264030..264281	-	252	WP_149201464.1	antitoxin	Antitoxin
HUE56_RS23435	264347..265051	-	705	WP_149201465.1	TetR/AcrR family transcriptional regulator	-
HUE56_RS23440	265302..266507	+	1206	WP_149201466.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB	1..443879	443879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (15-60)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14258.34 Da
• Isoelectric Point: 5.6651

>T159983 WP_149201463.1 NZ_CP054620:c264030-263632 [Azospirillum oryzae]
MLYMLDTNAMSDLVRNPSGKVAGHVRRVGEDAICTSIVVAAELRYGAAKKASAKLSRRVEDLLSDVAVLPFDLPADAEYG
SIRAELEAAGHPIGGNDLLIAAHARALDTILVTANMREFQRVRGLSVENWLE

Nucleotide

• Length: 399 bp

>T159983 NZ_CP070229:3913435-3913842 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGTGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9566.99 Da
• Isoelectric Point: 4.6936

>AT159983 WP_149201464.1 NZ_CP054620:c264281-264030 [Azospirillum oryzae]
MPQPAAPQRKEARLFRNNRSQAVRIPVEFELPGESVFISRDGDRLIIEPVRKKGLLALLESWEPLDEEFPEIEDKPVAPK
DIF

Nucleotide

• Length: 252 bp

>AT159983 NZ_CP070229:3913188-3913454 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA