Detailed information of TA system TA159977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 257855..258387
• Replicon: plasmid unnamed1
• Accession: NZ_CP054615
• Organism: Azospirillum oryzae strain KACC 14407

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HUE56_RS01000
• Protein ID: WP_149200390.1
• Coordinates: 257855..258178 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE56_RS01005
• Protein ID: WP_149200391.1
• Coordinates: 258175..258387 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE56_RS00985	253732..255369	+	1638	WP_149200473.1	HAMP domain-containing protein	-
HUE56_RS00990	255445..256578	-	1134	WP_149200388.1	Rieske 2Fe-2S domain-containing protein	-
HUE56_RS00995	256648..257796	-	1149	WP_149200389.1	glycoside hydrolase	-
HUE56_RS01000	257855..258178	-	324	WP_149200390.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE56_RS01005	258175..258387	-	213	WP_149200391.1	antitoxin MazE family protein	Antitoxin
HUE56_RS01010	258598..259587	+	990	WP_149200392.1	hypothetical protein	-
HUE56_RS01015	259618..260565	-	948	WP_149200393.1	glycosyltransferase family 2 protein	-
HUE56_RS01020	260579..262942	-	2364	WP_149200394.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / gmd	1..318077	318077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (8-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11606.48 Da
• Isoelectric Point: 8.0272

>T159977 WP_149200390.1 NZ_CP054615:c258178-257855 [Azospirillum oryzae]
VRRGDLVTVALQGDCGKPRPALVIQSDLFDGHPSVTILPITSALVDAPLFRPSVDPTPGNGLRKRSQVMVDKVQTIARDK
IGEAFGRLEDESMLTVNRAFLLWIGLA

Nucleotide

• Length: 324 bp

>T159977 NZ_CP070229:c2772201-2771890 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8215.30 Da
• Isoelectric Point: 8.0344

>AT159977 WP_149200391.1 NZ_CP054615:c258387-258175 [Azospirillum oryzae]
MPTPLSFRVQRHRNGLRAAGLRPLQIWVPDTRRPGFAAECRRQSLLVRDTPQEQETMDWLDAVRDTEGWE

Nucleotide

• Length: 213 bp

>AT159977 NZ_CP070229:c2771889-2771599 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG