Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 993892..994475 Replicon chromosome
Accession NZ_CP054527
Organism Bifidobacterium longum subsp. infantis strain BB-02

Toxin (Protein)


Gene name PumA Uniprot ID A0A1S2VZ23
Locus tag HUE60_RS04540 Protein ID WP_032745284.1
Coordinates 994173..994475 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I3AVV5
Locus tag HUE60_RS04535 Protein ID WP_007057517.1
Coordinates 993892..994176 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE60_RS04515 (HUE60_04490) 990398..991480 + 1083 Protein_879 transposase -
HUE60_RS04520 (HUE60_04495) 991578..992486 + 909 WP_193641239.1 ABC transporter ATP-binding protein -
HUE60_RS04525 (HUE60_04500) 992483..993214 + 732 WP_032744305.1 ABC transporter permease -
HUE60_RS04530 (HUE60_04505) 993232..993783 - 552 Protein_882 IS30 family transposase -
HUE60_RS04535 (HUE60_04510) 993892..994176 - 285 WP_007057517.1 putative addiction module antidote protein Antitoxin
HUE60_RS04540 (HUE60_04515) 994173..994475 - 303 WP_032745284.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUE60_RS04545 (HUE60_04520) 994513..994809 - 297 WP_032745282.1 hypothetical protein -
HUE60_RS04550 (HUE60_04525) 994968..995456 + 489 WP_032745281.1 D-aminoacyl-tRNA deacylase -
HUE60_RS04555 (HUE60_04530) 995617..998736 + 3120 WP_212104835.1 alpha-mannosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 986700..997553 10853
- flank IS/Tn - - 993232..993687 455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(9-92)

Antitoxin

Uncharacterized protein HI1420

(3-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11350.08 Da        Isoelectric Point: 10.4626

>T159852 WP_032745284.1 NZ_CP054527:c994475-994173 [Bifidobacterium longum subsp. infantis]
IKQSAEYRKWFKKLRDHKAKAAIQARLDACKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDKST
QQTDIRQAHDILNDYKEQRQ

Download         Length: 303 bp

>T159852 NZ_CP070157:3483857-3483964 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10158.37 Da        Isoelectric Point: 4.3707

>AT159852 WP_007057517.1 NZ_CP054527:c994176-993892 [Bifidobacterium longum subsp. infantis]
MSTEFTDYDTSEYLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Download         Length: 285 bp

>AT159852 NZ_CP070157:c3483808-3483745 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2VZ23

Antitoxin

Source ID Structure
AlphaFold DB I3AVV5

References