Detailed information of TA system TA159785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5743151..5743746
• Replicon: chromosome
• Accession: NZ_CP054473
• Organism: Pseudomonas aeruginosa strain PAAK095

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MS095_RS26725
• Protein ID: WP_003117425.1
• Coordinates: 5743468..5743746 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS095_RS26720
• Protein ID: WP_003099268.1
• Coordinates: 5743151..5743456 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS095_RS26690	5738604..5738894	-	291	WP_023083237.1	DUF5447 family protein	-
MS095_RS26695	5739106..5739378	-	273	WP_003115921.1	hypothetical protein	-
MS095_RS26700	5739488..5739754	+	267	WP_016852153.1	hypothetical protein	-
MS095_RS26705	5739928..5740722	+	795	WP_023082695.1	helix-turn-helix domain-containing protein	-
MS095_RS26710	5740697..5742235	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
MS095_RS26715	5742247..5742774	-	528	WP_071535723.1	AAA family ATPase	-
MS095_RS26720	5743151..5743456	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
MS095_RS26725	5743468..5743746	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS095_RS26730	5744075..5746303	+	2229	WP_003099265.1	TonB-dependent receptor	-
MS095_RS26735	5746373..5747020	-	648	WP_003095021.1	carbonate dehydratase	-
MS095_RS26740	5747082..5748320	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T159785 WP_003117425.1 NZ_CP054473:c5743746-5743468 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T159785 NZ_CP070134:c4914686-4914192 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT159785 WP_003099268.1 NZ_CP054473:c5743456-5743151 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT159785 NZ_CP070134:c4914976-4914683 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5