Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 878127..878781 Replicon chromosome
Accession NZ_CP054407
Organism Escherichia coli strain KCJ3K291

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag FR957_RS04315 Protein ID WP_000244772.1
Coordinates 878374..878781 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FR957_RS04310 Protein ID WP_000354046.1
Coordinates 878127..878393 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FR957_RS04285 (873296) 873296..874039 + 744 WP_000951964.1 SDR family oxidoreductase -
FR957_RS04290 (874096) 874096..875529 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FR957_RS04295 (875574) 875574..875885 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
FR957_RS04300 (876049) 876049..876708 + 660 WP_000250274.1 hemolysin III family protein -
FR957_RS04305 (876904) 876904..877884 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
FR957_RS04310 (878127) 878127..878393 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FR957_RS04315 (878374) 878374..878781 + 408 WP_000244772.1 protein YgfX Toxin
FR957_RS04320 (878821) 878821..879342 - 522 WP_032359754.1 flavodoxin FldB -
FR957_RS04325 (879454) 879454..880350 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FR957_RS04330 (880375) 880375..881085 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FR957_RS04335 (881091) 881091..882824 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T159664 WP_000244772.1 NZ_CP054407:878374-878781 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159664 NZ_CP070103:c3441289-3441182 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGGACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT159664 WP_000354046.1 NZ_CP054407:878127-878393 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159664 NZ_CP070103:3441337-3441403 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References