Detailed information of TA system TA159642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 23400..24057
• Replicon: plasmid pKC148K
• Accession: NZ_CP054405
• Organism: Klebsiella pneumoniae strain KCJ3K293

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FOH49_RS00870
• Protein ID: WP_000270043.1
• Coordinates: 23707..24057 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOH49_RS00865
• Protein ID: WP_000124640.1
• Coordinates: 23400..23702 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOH49_RS00820 (FOH49_000815)	19025..19453	+	429	WP_000591074.1	hypothetical protein	-
FOH49_RS00825 (FOH49_000820)	19510..19869	+	360	WP_000422768.1	hypothetical protein	-
FOH49_RS00830 (FOH49_000825)	19869..20315	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FOH49_RS00835 (FOH49_000830)	20312..20830	+	519	WP_000210756.1	nitrite reductase	-
FOH49_RS00840 (FOH49_000835)	20830..21060	+	231	WP_000972663.1	hypothetical protein	-
FOH49_RS00845 (FOH49_000840)	21047..21904	+	858	WP_001167032.1	hypothetical protein	-
FOH49_RS00850 (FOH49_000845)	22135..22662	+	528	WP_001236377.1	thermonuclease family protein	-
FOH49_RS00855 (FOH49_000850)	22720..22992	+	273	WP_001043047.1	HU family DNA-binding protein	-
FOH49_RS00860 (FOH49_000855)	23080..23373	+	294	WP_001239997.1	chromosome segregation protein ParM	-
FOH49_RS00865 (FOH49_000860)	23400..23702	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FOH49_RS00870 (FOH49_000865)	23707..24057	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOH49_RS00875 (FOH49_000870)	24220..24768	+	549	WP_001061574.1	transcriptional regulator	-
FOH49_RS00880 (FOH49_000880)	25109..25303	+	195	WP_000343597.1	hypothetical protein	-
FOH49_RS00885 (FOH49_000885)	25314..25685	+	372	WP_000516916.1	hypothetical protein	-
FOH49_RS00890 (FOH49_000890)	25678..26148	+	471	WP_001281821.1	hypothetical protein	-
FOH49_RS00895 (FOH49_000895)	26163..26498	-	336	WP_000683476.1	hypothetical protein	-
FOH49_RS00900 (FOH49_000900)	26595..27083	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FOH49_RS00905 (FOH49_000905)	27086..27583	+	498	WP_000062185.1	hypothetical protein	-
FOH49_RS00910 (FOH49_000910)	27798..28502	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FOH49_RS00915 (FOH49_000915)	28565..28858	-	294	Protein_41	tunicamycin resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / blaNDM-1 / aadA16 / dfrA27 / aac(6')-Ib-cr / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2	-	1..147761	147761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T159642 WP_000270043.1 NZ_CP054405:c24057-23707 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T159642 NZ_CP070098:4896022-4896312 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT159642 WP_000124640.1 NZ_CP054405:c23702-23400 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT159642 NZ_CP070098:4895790-4896032 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9