Detailed information of TA system TA159592

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5062990..5063825
• Replicon: chromosome
• Accession: NZ_CP054388
• Organism: Escherichia coli strain 1517k

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3K2XF06
• Locus tag: E2126_RS23990
• Protein ID: WP_001546496.1
• Coordinates: 5062990..5063367 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: E2126_RS23995
• Protein ID: WP_032216526.1
• Coordinates: 5063457..5063825 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E2126_RS23960	5058118..5059266	-	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-
E2126_RS23965	5059338..5060321	-	984	WP_001361242.1	KpsF/GutQ family sugar-phosphate isomerase	-
E2126_RS23970	5061132..5061302	-	171	Protein_4678	IS110 family transposase	-
E2126_RS23975	5061644..5062486	-	843	WP_087904844.1	DUF4942 domain-containing protein	-
E2126_RS23980	5062571..5062768	-	198	WP_087904847.1	DUF957 domain-containing protein	-
E2126_RS23985	5062844..5062993	-	150	Protein_4681	hypothetical protein	-
E2126_RS23990	5062990..5063367	-	378	WP_001546496.1	type IV toxin-antitoxin system toxin CbtA	Toxin
E2126_RS23995	5063457..5063825	-	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E2126_RS24000	5063844..5064489	-	646	Protein_4684	antitoxin of toxin-antitoxin stability system	-
E2126_RS24005	5064508..5064729	-	222	WP_064262053.1	DUF987 domain-containing protein	-
E2126_RS24010	5064792..5065268	-	477	WP_001186773.1	RadC family protein	-
E2126_RS24015	5065284..5065769	-	486	WP_032216512.1	antirestriction protein	-
E2126_RS24020	5065861..5066679	-	819	WP_134261073.1	DUF945 domain-containing protein	-
E2126_RS24025	5066769..5067002	-	234	WP_001278287.1	DUF905 family protein	-
E2126_RS24030	5067008..5067685	-	678	WP_001097301.1	hypothetical protein	-
E2126_RS24035	5067833..5068513	-	681	WP_128452457.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5061132..5061236	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13900.92 Da
• Isoelectric Point: 8.5163

>T159592 WP_001546496.1 NZ_CP054388:c5063367-5062990 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T159592 NZ_CP070088:340968-341070 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT159592 WP_032216526.1 NZ_CP054388:c5063825-5063457 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT159592 NZ_CP070088:c341077-340932 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A3K2XF06