Detailed information of TA system TA159536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 482629..483443
• Replicon: chromosome
• Accession: NZ_CP054371
• Organism: Escherichia coli strain SCU-316

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: HPE41_RS02335
• Protein ID: WP_001054376.1
• Coordinates: 483186..483443 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: HPE41_RS02330
• Protein ID: WP_001309181.1
• Coordinates: 482629..483174 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE41_RS02300	478320..479633	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
HPE41_RS02305	479645..479923	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HPE41_RS02310	479920..481041	-	1122	WP_000010829.1	M42 family metallopeptidase	-
HPE41_RS02315	481440..481658	-	219	Protein_445	hypothetical protein	-
HPE41_RS02320	481827..482375	-	549	WP_001519301.1	hypothetical protein	-
HPE41_RS02325	482412..482573	-	162	WP_001519302.1	hypothetical protein	-
HPE41_RS02330	482629..483174	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
HPE41_RS02335	483186..483443	-	258	WP_001054376.1	hypothetical protein	Toxin
HPE41_RS02340	483820..484065	-	246	Protein_450	GNAT family N-acetyltransferase	-
HPE41_RS02345	484435..485421	+	987	WP_001323209.1	hypothetical protein	-
HPE41_RS02350	486004..486984	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HPE41_RS02355	487049..488155	-	1107	WP_023568191.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T159536 WP_001054376.1 NZ_CP054371:c483443-483186 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T159536 NZ_CP070071:c3343030-3342857 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT159536 WP_001309181.1 NZ_CP054371:c483174-482629 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT159536 NZ_CP070071:c3343354-3343058 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8