Detailed information of TA system TA159502

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 120315..121146
• Replicon: chromosome
• Accession: NZ_CP054368
• Organism: Escherichia coli strain SCU-115

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HPE48_RS00595
• Protein ID: WP_000854814.1
• Coordinates: 120315..120689 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HPE48_RS00600
• Protein ID: WP_174143663.1
• Coordinates: 120778..121146 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPE48_RS00555	115712..116878	+	1167	WP_001296209.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HPE48_RS00560	116997..117470	+	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
HPE48_RS00565	117668..118726	+	1059	WP_001200890.1	FUSC family protein	-
HPE48_RS00570	118897..119226	+	330	WP_000450409.1	DUF496 family protein	-
HPE48_RS00575	119327..119509	-	183	WP_001016348.1	hypothetical protein	-
HPE48_RS00580	119563..119709	+	147	Protein_115	transposase domain-containing protein	-
HPE48_RS00585	119998..120111	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HPE48_RS00590	120124..120318	-	195	WP_000988601.1	hypothetical protein	-
HPE48_RS00595	120315..120689	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HPE48_RS00600	120778..121146	-	369	WP_174143663.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HPE48_RS00605	121226..121447	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HPE48_RS00610	121510..121986	-	477	WP_001186773.1	RadC family protein	-
HPE48_RS00615	122002..122475	-	474	WP_001385393.1	antirestriction protein	-
HPE48_RS00620	122738..123559	-	822	WP_001234710.1	DUF945 domain-containing protein	-
HPE48_RS00625	123780..124190	-	411	WP_000846704.1	hypothetical protein	-
HPE48_RS00630	124206..124883	-	678	WP_001362823.1	hypothetical protein	-
HPE48_RS00635	125019..126089	-	1071	WP_000102631.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T159502 WP_000854814.1 NZ_CP054368:c120689-120315 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T159502 NZ_CP070066:1159569-1159904 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.58 Da
• Isoelectric Point: 6.3139

>AT159502 WP_174143663.1 NZ_CP054368:c121146-120778 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLMQEGNRLHYLADRAGIRVLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT159502 NZ_CP070066:1159321-1159569 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50