Detailed information of TA system TA159186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5213..5772
• Replicon: plasmid p1
• Accession: NZ_CP054237
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: HR070_RS24560
• Protein ID: WP_000421272.1
• Coordinates: 5213..5488 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: HR070_RS24565
• Protein ID: WP_001387467.1
• Coordinates: 5488..5772 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS24530	1263..2003	-	741	WP_001066942.1	tyrosine-type recombinase/integrase	-
HR070_RS24535	2124..2306	-	183	WP_042065278.1	hypothetical protein	-
HR070_RS24540	2649..2956	-	308	Protein_3	type II toxin-antitoxin system ParD family antitoxin	-
HR070_RS24545	3449..4618	+	1170	WP_001072355.1	hypothetical protein	-
HR070_RS24550	4651..4779	+	129	Protein_5	IS481 family transposase	-
HR070_RS24555	4814..5107	-	294	WP_001105060.1	hypothetical protein	-
HR070_RS24560	5213..5488	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR070_RS24565	5488..5772	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
HR070_RS24570	6377..7129	+	753	WP_000562172.1	IclR family transcriptional regulator	-
HR070_RS24575	7175..8140	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
HR070_RS24580	8173..8553	-	381	WP_000710783.1	Rid family detoxifying hydrolase	-
HR070_RS24585	8578..9468	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
HR070_RS24590	9701..9895	-	195	WP_000796505.1	hypothetical protein	-
HR070_RS24595	9869..10309	+	441	Protein_14	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1	-	1..131660	131660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T159186 WP_000421272.1 NZ_CP054237:c5488-5213 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T159186 NZ_CP069974:127453-127869 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT159186 WP_001387467.1 NZ_CP054237:c5772-5488 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT159186 NZ_CP069974:127226-127456 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880