Detailed information of TA system TA159179

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3318682..3319516
• Replicon: chromosome
• Accession: NZ_CP054236
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HR070_RS16055
• Protein ID: WP_000854690.1
• Coordinates: 3318682..3319059 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: HR070_RS16060
• Protein ID: WP_001305076.1
• Coordinates: 3319148..3319516 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS16015	3313683..3314174	-	492	WP_001308552.1	DUF1097 domain-containing protein	-
HR070_RS16020	3314276..3314830	-	555	WP_001001909.1	molecular chaperone YcdY	-
HR070_RS16025	3314854..3315591	-	738	WP_000283664.1	zinc-binding phosphatase	-
HR070_RS16030	3315646..3316584	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
HR070_RS16040	3317071..3317898	-	828	WP_021517820.1	DUF4942 domain-containing protein	-
HR070_RS16045	3317983..3318180	-	198	WP_000839293.1	DUF957 domain-containing protein	-
HR070_RS16050	3318197..3318685	-	489	WP_000761699.1	hypothetical protein	-
HR070_RS16055	3318682..3319059	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HR070_RS16060	3319148..3319516	-	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HR070_RS16065	3319566..3320210	-	645	WP_000094916.1	hypothetical protein	-
HR070_RS16070	3320229..3320450	-	222	WP_000692315.1	DUF987 domain-containing protein	-
HR070_RS16075	3320513..3320989	-	477	WP_001186170.1	RadC family protein	-
HR070_RS16080	3321005..3321478	-	474	WP_128549590.1	antirestriction protein	-
HR070_RS16085	3321741..3322562	-	822	WP_001234710.1	DUF945 domain-containing protein	-
HR070_RS16090	3322741..3322955	-	215	Protein_3153	DUF905 family protein	-
HR070_RS16095	3322973..3323428	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	3310065..3344870	34805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T159179 WP_000854690.1 NZ_CP054236:c3319059-3318682 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T159179 NZ_CP069973:c3881380-3881093 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT159179 WP_001305076.1 NZ_CP054236:c3319516-3319148 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT159179 NZ_CP069973:c3881619-3881380 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8