Detailed information of TA system TA159159

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 1114656..1115068
• Replicon: chromosome
• Accession: NZ_CP054236
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: HR070_RS05410
• Protein ID: WP_000132630.1
• Coordinates: 1114656..1114997 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	1114992..1115068 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS05390	1109725..1111005	-	1281	WP_001304526.1	DUF445 domain-containing protein	-
HR070_RS05395	1111190..1112110	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
HR070_RS05400	1112338..1112418	+	81	WP_020233658.1	hypothetical protein	-
HR070_RS05405	1112516..1114609	+	2094	WP_000648234.1	DUF262 domain-containing protein	-
HR070_RS05410	1114656..1114997	-	342	WP_000132630.1	endoribonuclease SymE	Toxin
-	1114992..1115068	+	77	NuclAT_6	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_6	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_6	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_6	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_7	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_7	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_7	-	Antitoxin
-	1114992..1115068	+	77	NuclAT_7	-	Antitoxin
HR070_RS05415	1115218..1116987	-	1770	WP_000110076.1	restriction endonuclease subunit S	-
HR070_RS05420	1116987..1118456	-	1470	WP_001315223.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T159159 WP_000132630.1 NZ_CP054236:c1114997-1114656 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T159159 NZ_CP069973:547896-548360 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT159159 NZ_CP054236:1114992-1115068 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin