Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2026345..2027176 Replicon chromosome
Accession NZ_CP054230
Organism Escherichia coli strain EcPF14

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HR072_RS09625 Protein ID WP_000854814.1
Coordinates 2026345..2026719 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag HR072_RS09630 Protein ID WP_001285586.1
Coordinates 2026808..2027176 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR072_RS09585 2021741..2022907 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HR072_RS09590 2023026..2023499 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
HR072_RS09595 2023697..2024755 + 1059 WP_001200890.1 FUSC family protein -
HR072_RS09600 2024927..2025256 + 330 WP_000450409.1 DUF496 family protein -
HR072_RS09605 2025357..2025539 - 183 WP_001016348.1 hypothetical protein -
HR072_RS09610 2025593..2025739 + 147 Protein_1887 transposase domain-containing protein -
HR072_RS09615 2026028..2026141 - 114 WP_001161660.1 DUF957 domain-containing protein -
HR072_RS09620 2026154..2026348 - 195 WP_000988601.1 hypothetical protein -
HR072_RS09625 2026345..2026719 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
HR072_RS09630 2026808..2027176 - 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
HR072_RS09635 2027250..2027471 - 222 WP_000692323.1 DUF987 domain-containing protein -
HR072_RS09640 2027540..2028016 - 477 WP_001186170.1 RadC family protein -
HR072_RS09645 2028032..2028505 - 474 WP_000855076.1 antirestriction protein -
HR072_RS09650 2028768..2029589 - 822 WP_176316500.1 DUF945 domain-containing protein -
HR072_RS09655 2029810..2030220 - 411 WP_000846704.1 hypothetical protein -
HR072_RS09660 2030236..2030913 - 678 WP_001362823.1 hypothetical protein -
HR072_RS09665 2031049..2032119 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T159099 WP_000854814.1 NZ_CP054230:c2026719-2026345 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T159099 NZ_CP069957:3553888-3553995 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT159099 WP_001285586.1 NZ_CP054230:c2027176-2026808 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT159099 NZ_CP069957:c3553830-3553776 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References