Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3683550..3684337 Replicon chromosome
Accession NZ_CP054224
Organism Escherichia coli strain EcPF16

Toxin (Protein)


Gene name yeeV Uniprot ID A0A377E3D6
Locus tag HR074_RS17890 Protein ID WP_001194695.1
Coordinates 3683960..3684337 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2H9G3E7
Locus tag HR074_RS17885 Protein ID WP_000066236.1
Coordinates 3683550..3683909 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR074_RS17840 3679064..3679402 - 339 WP_001513762.1 IS3 family transposase -
HR074_RS17845 3679496..3679690 + 195 Protein_3496 hypothetical protein -
HR074_RS17850 3679753..3680295 + 543 WP_001104018.1 DUF4339 domain-containing protein -
HR074_RS17855 3680355..3680807 + 453 WP_001061894.1 hypothetical protein -
HR074_RS17860 3680884..3681117 + 234 WP_001114682.1 DUF905 domain-containing protein -
HR074_RS17865 3681237..3682055 + 819 WP_001234406.1 DUF945 domain-containing protein -
HR074_RS17870 3682323..3682793 + 471 WP_000131762.1 antirestriction protein -
HR074_RS17875 3682805..3683284 + 480 WP_000437750.1 DNA repair protein RadC -
HR074_RS17880 3683305..3683526 + 222 WP_000691981.1 DUF987 domain-containing protein -
HR074_RS17885 3683550..3683909 + 360 WP_000066236.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HR074_RS17890 3683960..3684337 + 378 WP_001194695.1 TA system toxin CbtA family protein Toxin
HR074_RS17895 3684334..3684825 + 492 WP_000777682.1 hypothetical protein -
HR074_RS17900 3684857..3685060 + 204 WP_000413747.1 DUF957 domain-containing protein -
HR074_RS17905 3685141..3685985 + 845 Protein_3508 DUF4942 domain-containing protein -
HR074_RS17915 3686327..3687058 - 732 WP_001300756.1 DNA polymerase III subunit epsilon -
HR074_RS17920 3687123..3687590 + 468 WP_000917883.1 ribonuclease HI -
HR074_RS17925 3687587..3688309 - 723 WP_001295200.1 class I SAM-dependent methyltransferase -
HR074_RS17930 3688343..3689098 + 756 WP_001052721.1 hydroxyacylglutathione hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3679753..3689098 9345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14180.97 Da        Isoelectric Point: 7.4209

>T159055 WP_001194695.1 NZ_CP054224:3683960-3684337 [Escherichia coli]
MQTQPLSSTQEATSRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKATGLMTRNDYRTVTDITTGKYRGGHR

Download         Length: 378 bp

>T159055 NZ_CP069945:c3698793-3698458 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13127.02 Da        Isoelectric Point: 7.0261

>AT159055 WP_000066236.1 NZ_CP054224:3683550-3683909 [Escherichia coli]
MSNKIPTVNHDITEPWWGLKRSITPCFGARLVQSGNCLHYLADRASITGQFSDGDLRHLDQAFPLLLKQLELMLTSGELT
PRHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT159055 NZ_CP069945:c3699041-3698793 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377E3D6

Antitoxin

Source ID Structure
AlphaFold DB A0A2H9G3E7

References