Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2549360..2549998 Replicon chromosome
Accession NZ_CP054224
Organism Escherichia coli strain EcPF16

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HR074_RS12330 Protein ID WP_000813794.1
Coordinates 2549360..2549536 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HR074_RS12335 Protein ID WP_001270286.1
Coordinates 2549582..2549998 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR074_RS12310 2544979..2546193 - 1215 WP_001326689.1 BenE family transporter YdcO -
HR074_RS12315 2546246..2546782 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HR074_RS12320 2546855..2548816 + 1962 WP_032238462.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HR074_RS12325 2548908..2549138 - 231 WP_000494244.1 YncJ family protein -
HR074_RS12330 2549360..2549536 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HR074_RS12335 2549582..2549998 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HR074_RS12340 2550077..2551483 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HR074_RS12345 2551728..2552873 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HR074_RS12350 2552891..2553904 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
HR074_RS12355 2553905..2554846 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T159049 WP_000813794.1 NZ_CP054224:2549360-2549536 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T159049 NZ_CP069945:c2342523-2342245 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGAAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT159049 WP_001270286.1 NZ_CP054224:2549582-2549998 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT159049 NZ_CP069945:c2342245-2341961 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References