Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 48924..49603 Replicon chromosome
Accession NZ_CP054224
Organism Escherichia coli strain EcPF16

Toxin (Protein)


Gene name ykfI Uniprot ID A0A377N7M4
Locus tag HR074_RS00240 Protein ID WP_000804348.1
Coordinates 48924..49265 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A377N5M0
Locus tag HR074_RS00245 Protein ID WP_000065827.1
Coordinates 49286..49603 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR074_RS00215 44426..44719 - 294 WP_000805509.1 hypothetical protein -
HR074_RS00220 44941..45759 + 819 WP_000779426.1 lipoprotein NlpA -
HR074_RS00225 45763..46686 - 924 WP_000535960.1 carboxylate/amino acid/amine transporter -
HR074_RS00230 46797..47981 - 1185 WP_001172876.1 sugar efflux transporter -
HR074_RS00235 48363..48509 - 147 WP_001315909.1 hypothetical protein -
HR074_RS00240 48924..49265 - 342 WP_000804348.1 TA system toxin CbtA family protein Toxin
HR074_RS00245 49286..49603 - 318 WP_000065827.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HR074_RS00250 49622..49843 - 222 WP_000691993.1 DUF987 domain-containing protein -
HR074_RS00255 49852..50328 - 477 WP_000811695.1 RadC family protein -
HR074_RS00260 50344..50802 - 459 WP_000211840.1 antirestriction protein -
HR074_RS00265 50903..51142 - 240 WP_000083670.1 DUF905 domain-containing protein -
HR074_RS00270 51219..51686 - 468 WP_001446317.1 hypothetical protein -
HR074_RS00275 51710..52153 - 444 WP_000824224.1 hypothetical protein -
HR074_RS00280 52153..52389 - 237 WP_001115854.1 hypothetical protein -
HR074_RS00285 52426..53127 - 702 WP_000189410.1 WYL domain-containing protein -
HR074_RS00290 53344..54165 - 822 WP_000197394.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12914.00 Da        Isoelectric Point: 10.2232

>T159034 WP_000804348.1 NZ_CP054224:c49265-48924 [Escherichia coli]
MKPQSATISRAAKPCLSPVAVWQMLLIRLLEKHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRRNRKHTAL

Download         Length: 342 bp

>T159034 NZ_CP069943:1934061-1934471 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAACGGCGGATCCATGCCCGACAGGGGGAAATAAAGTTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGTCTGCGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12003.68 Da        Isoelectric Point: 6.9464

>AT159034 WP_000065827.1 NZ_CP054224:c49603-49286 [Escherichia coli]
MSNITWGLQRDITPRLGVRLVQEGNRLHYLADRASITGKFSDPECRKLDETFPHFISQMELMLTTGELNPRHTHCIALYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT159034 NZ_CP069943:1933814-1934080 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377N7M4

Antitoxin

Source ID Structure
AlphaFold DB A0A377N5M0

References