Detailed information of TA system TA158986

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41471..41735
• Replicon: plasmid p2
• Accession: NZ_CP054216
• Organism: Escherichia coli strain EcPF40

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: HR076_RS25090
• Protein ID: WP_042078455.1
• Coordinates: 41583..41735 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	41471..41528 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR076_RS25075	36710..39001	-	2292	WP_077877997.1	conjugal transfer protein TrbC	-
HR076_RS25080	38994..40064	-	1071	WP_012783932.1	IncI1-type conjugal transfer protein TrbB	-
HR076_RS25085	40083..41291	-	1209	WP_042078447.1	IncI1-type conjugal transfer protein TrbA	-
-	41471..41528	-	58	NuclAT_0	-	Antitoxin
-	41471..41528	-	58	NuclAT_0	-	Antitoxin
-	41471..41528	-	58	NuclAT_0	-	Antitoxin
-	41471..41528	-	58	NuclAT_0	-	Antitoxin
HR076_RS25090	41583..41735	+	153	WP_042078455.1	Hok/Gef family protein	Toxin
HR076_RS25095	41807..42058	-	252	WP_023225283.1	hypothetical protein	-
HR076_RS25100	42717..42893	-	177	WP_001054898.1	hypothetical protein	-
HR076_RS25105	43225..43434	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HR076_RS25110	43506..44168	-	663	WP_042078443.1	plasmid IncI1-type surface exclusion protein ExcA	-
HR076_RS25115	44238..46406	-	2169	WP_042078442.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88657	88657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5789.15 Da
• Isoelectric Point: 8.7948

>T158986 WP_042078455.1 NZ_CP054216:41583-41735 [Escherichia coli]
MPQRTFLMMLIVICVTILCFIWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T158986 NZ_CP069935:c151570-151352 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT158986 NZ_CP054216:c41528-41471 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin