Detailed information of TA system TA158935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 163751..164376
• Replicon: plasmid pHNTH9F11-1
• Accession: NZ_CP054192
• Organism: Escherichia coli strain TH9F11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HRL54_RS24495
• Protein ID: WP_000911324.1
• Coordinates: 163978..164376 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: HRL54_RS24490
• Protein ID: WP_000450532.1
• Coordinates: 163751..163978 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRL54_RS24490 (163751)	163751..163978	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HRL54_RS24495 (163978)	163978..164376	+	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
HRL54_RS24500 (164385)	164385..166538	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
HRL54_RS24505 (166791)	166791..167522	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
HRL54_RS24510 (167547)	167547..168068	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / tet(A) / blaCTX-M-55 / fosA3 / dfrA1 / sitABCD / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR	iroB / iroC / iroD / iroE / iroN	1..407456	407456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T158935 WP_000911324.1 NZ_CP054192:163978-164376 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T158935 NZ_CP069891:25952-26257 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GTCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT158935 WP_000450532.1 NZ_CP054192:163751-163978 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT158935 NZ_CP069891:25732-25950 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5