Detailed information of TA system TA158661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1498032..1498687
• Replicon: chromosome
• Accession: NZ_CP054067
• Organism: Bordetella pertussis strain NZ64

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: HRK18_RS07320
• Protein ID: WP_047122780.1
• Coordinates: 1498032..1498283 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q7VY40
• Locus tag: HRK18_RS07325
• Protein ID: WP_003809516.1
• Coordinates: 1498286..1498687 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRK18_RS07295 (HRK18_07255)	1493193..1494323	-	1131	WP_010930401.1	helix-turn-helix transcriptional regulator	-
HRK18_RS07300 (HRK18_07260)	1494558..1495139	+	582	WP_019247931.1	MOSC domain-containing protein	-
HRK18_RS07305 (HRK18_07265)	1495276..1496340	+	1065	WP_003809510.1	fructose-bisphosphate aldolase class II	-
HRK18_RS07310 (HRK18_07270)	1496425..1496871	-	447	WP_003819827.1	GFA family protein	-
HRK18_RS07315 (HRK18_07275)	1497096..1497977	+	882	WP_003809513.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HRK18_RS07320 (HRK18_07280)	1498032..1498283	+	252	WP_047122780.1	type II toxin-antitoxin system MqsR family toxin	Toxin
HRK18_RS07325 (HRK18_07285)	1498286..1498687	+	402	WP_003809516.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
HRK18_RS07330 (HRK18_07290)	1498707..1498943	+	237	WP_010930404.1	DUF5993 family protein	-
HRK18_RS07335 (HRK18_07295)	1498951..1499430	+	480	WP_010930405.1	disulfide bond formation protein B	-
HRK18_RS07340 (HRK18_07300)	1499580..1500095	+	516	WP_019247930.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
HRK18_RS07345 (HRK18_07305)	1500098..1501288	+	1191	WP_010930406.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
HRK18_RS07350 (HRK18_07310)	1501308..1502345	+	1038	WP_023853543.1	L-threonylcarbamoyladenylate synthase	-
HRK18_RS07355 (HRK18_07315)	1502482..1503504	+	1023	WP_003809532.1	amino acid ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (10-82)

Antitoxin

• Antitoxin MqsA/HigA-2 (1-131)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 8952.52 Da
• Isoelectric Point: 9.7242

>T158661 WP_047122780.1 NZ_CP054067:1498032-1498283 [Bordetella pertussis]
IGGAATKKPGQKRRAGAAALGLDFSAMVEIVLALTPRDFHKSMTTHADHRIWQDVYCPVTAMGAVYLKLTVLDGVPIVSF
KEL

Nucleotide

• Length: 252 bp

>T158661 NZ_CP069709:1930329-1930432 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14528.70 Da
• Isoelectric Point: 8.5005

>AT158661 WP_003809516.1 NZ_CP054067:1498286-1498687 [Bordetella pertussis]
MKCPVCGAAALARDTRDVPYSYKGETITIAAVTGDWCPACGENVLDGAESARVSEAMLAFNRLVNARRVDPGFIVRVRRK
LGLDQREAAALFGGGTNAFSRYENGKTRPPLALVKLLRLLDRHPELLEEVRAD

Nucleotide

• Length: 402 bp

>AT158661 NZ_CP069709:c1930470-1930199 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A0H3LKS6