Detailed information of TA system TA158632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1982485..1983264
• Replicon: chromosome
• Accession: NZ_CP054060
• Organism: Edwardsiella ictaluri strain T1-1

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: F8539_RS09395
• Protein ID: WP_198077053.1
• Coordinates: 1982485..1982742 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: C5BBM9
• Locus tag: F8539_RS09400
• Protein ID: WP_015871334.1
• Coordinates: 1982752..1983264 (+)
• Length: 171 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F8539_RS09365	1977680..1977924	-	245	Protein_1801	transposase	-
F8539_RS09370	1978030..1978848	-	819	WP_198077051.1	hypothetical protein	-
F8539_RS09375	1978913..1980253	-	1341	WP_049640648.1	Fic family protein	-
F8539_RS09380	1980382..1981152	-	771	WP_198077052.1	Rha family transcriptional regulator	-
F8539_RS09385	1981607..1981936	+	330	WP_015871332.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F8539_RS09390	1981926..1982234	+	309	WP_015871333.1	helix-turn-helix transcriptional regulator	-
F8539_RS09395	1982485..1982742	+	258	WP_198077053.1	DUF4160 domain-containing protein	Toxin
F8539_RS09400	1982752..1983264	+	513	WP_015871334.1	DUF2442 domain-containing protein	Antitoxin
F8539_RS09405	1983336..1983635	-	300	WP_049640646.1	XRE family transcriptional regulator	-
F8539_RS09410	1983637..1983987	-	351	WP_198077054.1	type II toxin-antitoxin system RelE/ParE family toxin	-
F8539_RS09415	1984037..1984348	-	312	WP_049640644.1	DUF1364 domain-containing protein	-
F8539_RS09420	1984351..1984995	-	645	WP_198077055.1	DUF1367 family protein	-
F8539_RS09425	1985105..1985452	-	348	WP_015871339.1	hypothetical protein	-
F8539_RS09430	1985482..1986864	-	1383	WP_049640642.1	replicative DNA helicase	-
F8539_RS09435	1986861..1987811	-	951	WP_198077056.1	helix-turn-helix domain-containing protein	-
F8539_RS09440	1987815..1988003	-	189	WP_198077057.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1936155..2004122	67967

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (81-161)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9625.29 Da
• Isoelectric Point: 7.4665

>T158632 WP_198077053.1 NZ_CP054060:1982485-1982742 [Edwardsiella ictaluri]
MPEIDALMGLSFVMYFFDNQTHSLPHVHVKYGEFELVIGIDSGEALEGYLPPKKRKLAESHIKANKTRLLAMWQMAVNGM
HPGKL

Nucleotide

• Length: 258 bp

>T158632 NZ_CP069706:3717239-3717517 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 171 a.a.
• Molecular weight: 18155.52 Da
• Isoelectric Point: 7.3201

>AT158632 WP_015871334.1 NZ_CP054060:1982752-1983264 [Edwardsiella ictaluri]
MLKIIDVDVVGDHVIEVEFSDGFRGRADLAALFSKPPFSAIADFNRFSLTASGVLNWGDAELSADTVRRISKGAVVSASS
RALTPENVEAILRQATWESMSEGRPDILQAALRGYAEQLGHADVIKRAGIASRSSAYKTLSPSTNPSFKSLAKISGAILA
IVRENNAQHG

Nucleotide

• Length: 513 bp

>AT158632 NZ_CP069706:3716976-3717236 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: C5BBM9