Detailed information of TA system TA158308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 522980..523633
• Replicon: chromosome
• Accession: NZ_CP053927
• Organism: Serratia marcescens strain 11/2010

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HP437_RS03220
• Protein ID: WP_060440839.1
• Coordinates: 522980..523330 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: HP437_RS03225
• Protein ID: WP_019453270.1
• Coordinates: 523334..523633 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP437_RS03190	519480..519797	+	318	WP_102985510.1	hypothetical protein	-
HP437_RS03195	519788..520183	-	396	WP_180548076.1	hypothetical protein	-
HP437_RS03200	520197..520589	-	393	WP_102985508.1	hypothetical protein	-
HP437_RS03205	520617..521006	-	390	WP_084826902.1	hypothetical protein	-
HP437_RS03210	521006..522010	-	1005	WP_180548077.1	hypothetical protein	-
HP437_RS03215	522121..522573	-	453	WP_180548078.1	hypothetical protein	-
HP437_RS03220	522980..523330	+	351	WP_060440839.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HP437_RS03225	523334..523633	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
HP437_RS03230	523623..524540	-	918	WP_049272489.1	LysR family transcriptional regulator	-
HP437_RS03235	524709..525407	+	699	WP_049212496.1	CoA transferase subunit A	-
HP437_RS03240	525419..526072	+	654	WP_019453268.1	CoA transferase subunit B	-
HP437_RS03245	526086..527270	+	1185	WP_180548079.1	acetyl-CoA C-acetyltransferase	-
HP437_RS03250	527289..528212	+	924	WP_060422138.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13536.77 Da
• Isoelectric Point: 8.2699

>T158308 WP_060440839.1 NZ_CP053927:522980-523330 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGSLHSNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T158308 NZ_CP069621:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT158308 WP_019453270.1 NZ_CP053927:523334-523633 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT158308 NZ_CP069621:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3