Detailed information of TA system TA158021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 446565..447180
• Replicon: chromosome
• Accession: NZ_CP053814
• Organism: Vibrio cholerae strain W7G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: HPY09_RS15865
• Protein ID: WP_001162670.1
• Coordinates: 446565..446852 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HPY09_RS15870
• Protein ID: WP_001232701.1
• Coordinates: 446863..447180 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY09_RS15825	441642..442280	+	639	WP_002051085.1	HD domain-containing protein	-
HPY09_RS15830	442439..442852	+	414	WP_175247191.1	GNAT family N-acetyltransferase	-
HPY09_RS15835	442963..443325	+	363	WP_162796981.1	DUF4144 domain-containing protein	-
HPY09_RS15840	443469..443939	+	471	WP_175247100.1	hypothetical protein	-
HPY09_RS15845	444255..444410	+	156	WP_071170622.1	DUF645 family protein	-
HPY09_RS15850	444617..445069	+	453	WP_175247192.1	GNAT family N-acetyltransferase	-
HPY09_RS15855	445216..445812	+	597	WP_175247193.1	class I SAM-dependent methyltransferase	-
HPY09_RS15860	446129..446284	+	156	WP_108911962.1	DUF645 family protein	-
HPY09_RS15865	446565..446852	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY09_RS15870	446863..447180	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
HPY09_RS15875	447307..447819	-	513	WP_175247194.1	hypothetical protein	-
HPY09_RS15880	447858..447974	+	117	Protein_479	DUF3265 domain-containing protein	-
HPY09_RS15885	448160..448342	+	183	WP_175247341.1	DUF645 family protein	-
HPY09_RS15890	448719..448874	+	156	WP_071170622.1	DUF645 family protein	-
HPY09_RS15895	449075..449785	+	711	WP_175247195.1	hypothetical protein	-
HPY09_RS15900	449778..449891	+	114	WP_175247196.1	DUF3265 domain-containing protein	-
HPY09_RS15905	449923..450555	+	633	WP_001894947.1	HNH endonuclease	-
HPY09_RS15910	450676..451131	+	456	WP_001245329.1	GNAT family N-acetyltransferase	-
HPY09_RS15915	451294..451845	+	552	WP_175247197.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	317876..527757	209881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T158021 WP_001162670.1 NZ_CP053814:446565-446852 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T158021 NZ_CP069542:4256860-4257138 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT158021 WP_001232701.1 NZ_CP053814:446863-447180 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT158021 NZ_CP069542:4256597-4256857 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36