Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3407047..3407701 Replicon chromosome
Accession NZ_CP053736
Organism Escherichia coli strain CP8-3_Sichuan

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HP430_RS16385 Protein ID WP_000244777.1
Coordinates 3407047..3407454 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HP430_RS16390 Protein ID WP_000354046.1
Coordinates 3407435..3407701 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP430_RS16365 3403004..3404737 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
HP430_RS16370 3404743..3405453 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HP430_RS16375 3405478..3406374 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HP430_RS16380 3406486..3407007 + 522 WP_001055874.1 flavodoxin FldB -
HP430_RS16385 3407047..3407454 - 408 WP_000244777.1 toxin CptA Toxin
HP430_RS16390 3407435..3407701 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HP430_RS16395 3407944..3408924 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HP430_RS16400 3409120..3409779 - 660 WP_000250274.1 hemolysin III family protein -
HP430_RS16405 3409943..3410254 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HP430_RS16410 3410299..3411732 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HP430_RS16415 3411789..3412532 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T157670 WP_000244777.1 NZ_CP053736:c3407454-3407047 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T157670 NZ_CP069445:c2227681-2227346 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT157670 WP_000354046.1 NZ_CP053736:c3407701-3407435 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT157670 NZ_CP069445:c2227929-2227681 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References