Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2920853..2921491 Replicon chromosome
Accession NZ_CP053730
Organism Escherichia coli strain CP61_Sichuan

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HP432_RS14975 Protein ID WP_000813794.1
Coordinates 2920853..2921029 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HP432_RS14980 Protein ID WP_001270286.1
Coordinates 2921075..2921491 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP432_RS14955 2916472..2917686 - 1215 WP_071597387.1 BenE family transporter YdcO -
HP432_RS14960 2917739..2918275 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
HP432_RS14965 2918348..2920309 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HP432_RS14970 2920401..2920631 - 231 WP_000494244.1 YncJ family protein -
HP432_RS14975 2920853..2921029 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HP432_RS14980 2921075..2921491 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HP432_RS14985 2921570..2922976 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
HP432_RS14990 2923221..2924366 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HP432_RS14995 2924384..2925397 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
HP432_RS15000 2925398..2926339 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2915167..2916432 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T157600 WP_000813794.1 NZ_CP053730:2920853-2921029 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T157600 NZ_CP069386:c3208628-3208287 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT157600 WP_001270286.1 NZ_CP053730:2921075-2921491 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT157600 NZ_CP069386:3208623-3208699 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References