157076

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2743776..2745213 Replicon chromosome
Accession NZ_CP053603
Organism Escherichia coli strain Dam/Dcm

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag HO395_RS13275 Protein ID WP_000155570.1
Coordinates 2743776..2744849 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag HO395_RS13280 Protein ID WP_000461704.1
Coordinates 2744842..2745213 (+) Length 124 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO395_RS13260 2739209..2741398 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
HO395_RS13265 2741395..2743011 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
HO395_RS13270 2743371..2743634 - 264 WP_000502050.1 DUF1524 domain-containing protein -
HO395_RS13275 2743776..2744849 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
HO395_RS13280 2744842..2745213 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
HO395_RS13285 2745568..2746431 + 864 WP_000787597.1 GTPase family protein -
HO395_RS13290 2746523..2747344 + 822 WP_000197391.1 DUF945 domain-containing protein -
HO395_RS13295 2747561..2748262 + 702 WP_000189409.1 WYL domain-containing protein -
HO395_RS13300 2748303..2748539 + 237 WP_001144029.1 hypothetical protein -
HO395_RS13305 2748539..2748982 + 444 WP_000824222.1 hypothetical protein -
HO395_RS13310 2749006..2749473 + 468 WP_000702495.1 hypothetical protein -
HO395_RS13315 2749698..2750012 - 315 WP_000065803.1 arsenate reductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T157076 WP_000155570.1 NZ_CP053603:2743776-2744849 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T157076 NZ_CP069076:c637113-636700 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT157076 WP_000461704.1 NZ_CP053603:2744842-2745213 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT157076 NZ_CP069076:c637376-637110 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S


Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References