Detailed information of TA system TA157076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2743776..2745213
• Replicon: chromosome
• Accession: NZ_CP053603
• Organism: Escherichia coli strain Dam/Dcm

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: HO395_RS13275
• Protein ID: WP_000155570.1
• Coordinates: 2743776..2744849 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: HO395_RS13280
• Protein ID: WP_000461704.1
• Coordinates: 2744842..2745213 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO395_RS13260	2739209..2741398	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
HO395_RS13265	2741395..2743011	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
HO395_RS13270	2743371..2743634	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
HO395_RS13275	2743776..2744849	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
HO395_RS13280	2744842..2745213	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
HO395_RS13285	2745568..2746431	+	864	WP_000787597.1	GTPase family protein	-
HO395_RS13290	2746523..2747344	+	822	WP_000197391.1	DUF945 domain-containing protein	-
HO395_RS13295	2747561..2748262	+	702	WP_000189409.1	WYL domain-containing protein	-
HO395_RS13300	2748303..2748539	+	237	WP_001144029.1	hypothetical protein	-
HO395_RS13305	2748539..2748982	+	444	WP_000824222.1	hypothetical protein	-
HO395_RS13310	2749006..2749473	+	468	WP_000702495.1	hypothetical protein	-
HO395_RS13315	2749698..2750012	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T157076 WP_000155570.1 NZ_CP053603:2743776-2744849 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T157076 NZ_CP069076:c637113-636700 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT157076 WP_000461704.1 NZ_CP053603:2744842-2745213 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT157076 NZ_CP069076:c637376-637110 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• AlphaFold DB: A0A373F4G4