156907

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3990978..3991815 Replicon chromosome
Accession NZ_CP053592
Organism Escherichia coli strain NEBExpress_Iq

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag HO403_RS19270 Protein ID WP_000227786.1
Coordinates 3991273..3991815 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HO403_RS19265 Protein ID WP_001297137.1
Coordinates 3990978..3991289 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO403_RS19240 3985998..3986945 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
HO403_RS19245 3986967..3988958 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HO403_RS19250 3988948..3989562 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HO403_RS19255 3989562..3989891 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HO403_RS19260 3989903..3990793 + 891 WP_000971336.1 heme o synthase -
HO403_RS19265 3990978..3991289 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HO403_RS19270 3991273..3991815 + 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
HO403_RS19275 3991871..3992806 - 936 WP_001297127.1 sel1 repeat family protein -
HO403_RS19280 3993223..3994587 + 1365 WP_001000978.1 MFS transporter -
HO403_RS19285 3994715..3995206 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HO403_RS19290 3995374..3996285 + 912 WP_000705853.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T156907 WP_000227786.1 NZ_CP053592:3991273-3991815 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T156907 NZ_CP069073:721644-722039 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT156907 WP_001297137.1 NZ_CP053592:3990978-3991289 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT156907 NZ_CP069073:721390-721644 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References