Detailed information of TA system TA156606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3309716..3310248
• Replicon: chromosome
• Accession: NZ_CP053300
• Organism: Burkholderia cenocepacia strain K56-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B4EDC8
• Locus tag: K562_RS15505
• Protein ID: WP_006484901.1
• Coordinates: 3309925..3310248 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B4EDC7
• Locus tag: K562_RS15500
• Protein ID: WP_012492929.1
• Coordinates: 3309716..3309928 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K562_RS15465	3305059..3305571	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
K562_RS15470	3305591..3306340	+	750	WP_006484965.1	tol-pal system protein YbgF	-
K562_RS15480	3306768..3307088	-	321	WP_012492928.1	helix-turn-helix domain-containing protein	-
K562_RS15485	3307227..3307622	+	396	WP_006484952.1	hypothetical protein	-
K562_RS15490	3307690..3308142	+	453	WP_006484947.1	hypothetical protein	-
K562_RS15495	3308410..3309429	+	1020	WP_012492245.1	IS110-like element ISBcen5 family transposase	-
K562_RS15500	3309716..3309928	+	213	WP_012492929.1	antitoxin MazE family protein	Antitoxin
K562_RS15505	3309925..3310248	+	324	WP_006484901.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K562_RS15510	3310568..3311092	+	525	WP_012492930.1	hypothetical protein	-
K562_RS15515	3311094..3312119	+	1026	WP_012492931.1	relaxase/mobilization nuclease domain-containing protein	-
K562_RS15520	3312242..3312685	-	444	WP_011544671.1	YiaA/YiaB family protein	-
K562_RS15525	3312789..3313736	-	948	WP_006484950.1	AraC family transcriptional regulator	-
K562_RS15530	3313866..3314195	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
K562_RS15535	3314396..3315229	+	834	WP_034195721.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3306768..3315991	9223
	-	flank	IS/Tn	-	-	3308410..3309429	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11652.77 Da
• Isoelectric Point: 8.9366

>T156606 WP_006484901.1 NZ_CP053300:3309925-3310248 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T156606 NZ_CP069068:c642734-642321 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.99 Da
• Isoelectric Point: 5.5492

>AT156606 WP_012492929.1 NZ_CP053300:3309716-3309928 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQVTDLGHSQ

Nucleotide

• Length: 213 bp

>AT156606 NZ_CP069068:c642997-642731 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: B4EDC8

Antitoxin

• AlphaFold DB: B4EDC7