Detailed information of TA system TA156525

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1717851..1718695
• Replicon: chromosome
• Accession: NZ_CP053284
• Organism: Escherichia coli strain SCU-104

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: HHJ22_RS08205
• Protein ID: WP_000854686.1
• Coordinates: 1718312..1718695 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: HHJ22_RS08200
• Protein ID: WP_001285602.1
• Coordinates: 1717851..1718231 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ22_RS08160	1713306..1713514	+	209	Protein_1595	DUF905 family protein	-
HHJ22_RS08165	1713615..1714436	+	822	WP_096929955.1	DUF945 domain-containing protein	-
HHJ22_RS08170	1714528..1715013	+	486	WP_000214307.1	antirestriction protein	-
HHJ22_RS08175	1715029..1715505	+	477	WP_001186726.1	RadC family protein	-
HHJ22_RS08180	1715568..1715789	+	222	WP_000692298.1	DUF987 domain-containing protein	-
HHJ22_RS08185	1715808..1716110	+	303	Protein_1600	hypothetical protein	-
HHJ22_RS08190	1716178..1717290	+	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
HHJ22_RS08195	1717463..1717840	+	378	WP_000271247.1	hypothetical protein	-
HHJ22_RS08200	1717851..1718231	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ22_RS08205	1718312..1718695	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
HHJ22_RS08210	1718692..1719180	+	489	WP_001054233.1	hypothetical protein	-
HHJ22_RS08215	1719197..1719394	+	198	WP_000839253.1	DUF957 domain-containing protein	-
HHJ22_RS08220	1719479..1720321	+	843	WP_001431817.1	DUF4942 domain-containing protein	-
HHJ22_RS08230	1720812..1721030	-	219	WP_001040187.1	translation initiation factor IF-1	-
HHJ22_RS08235	1721315..1722019	-	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T156525 WP_000854686.1 NZ_CP053284:1718312-1718695 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T156525 NZ_CP069066:c2844319-2843900 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT156525 WP_001285602.1 NZ_CP053284:1717851-1718231 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT156525 NZ_CP069066:c2844540-2844316 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGTTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA