Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1166966..1167584 Replicon chromosome
Accession NZ_CP053284
Organism Escherichia coli strain SCU-104

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HHJ22_RS05535 Protein ID WP_001291435.1
Coordinates 1166966..1167184 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HHJ22_RS05540 Protein ID WP_000344800.1
Coordinates 1167210..1167584 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ22_RS05500 1162256..1162828 + 573 WP_000779831.1 YbaY family lipoprotein -
HHJ22_RS05505 1162859..1163170 - 312 WP_000409911.1 MGMT family protein -
HHJ22_RS05515 1163549..1163902 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HHJ22_RS05520 1163944..1165494 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HHJ22_RS05525 1165658..1166128 - 471 WP_000136192.1 YlaC family protein -
HHJ22_RS05530 1166244..1166795 - 552 WP_000102564.1 maltose O-acetyltransferase -
HHJ22_RS05535 1166966..1167184 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HHJ22_RS05540 1167210..1167584 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HHJ22_RS05545 1168130..1171279 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HHJ22_RS05550 1171302..1172495 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T156523 WP_001291435.1 NZ_CP053284:c1167184-1166966 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T156523 NZ_CP069066:c2396728-2396411 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTTCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT156523 WP_000344800.1 NZ_CP053284:c1167584-1167210 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT156523 NZ_CP069066:c2396940-2396725 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References