Detailed information of TA system TA156509

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3090417..3091261
• Replicon: chromosome
• Accession: NZ_CP053281
• Organism: Escherichia coli strain SCU-308

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: HHJ45_RS14975
• Protein ID: WP_000854686.1
• Coordinates: 3090417..3090800 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: HHJ45_RS14980
• Protein ID: WP_001285602.1
• Coordinates: 3090881..3091261 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ45_RS14945	3087093..3087797	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
HHJ45_RS14950	3088082..3088300	+	219	WP_001040187.1	translation initiation factor IF-1	-
HHJ45_RS14960	3088791..3089633	-	843	WP_074767346.1	DUF4942 domain-containing protein	-
HHJ45_RS14965	3089718..3089915	-	198	WP_000839253.1	DUF957 domain-containing protein	-
HHJ45_RS14970	3089932..3090420	-	489	WP_001054233.1	hypothetical protein	-
HHJ45_RS14975	3090417..3090800	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
HHJ45_RS14980	3090881..3091261	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HHJ45_RS14985	3091272..3091955	-	684	WP_000086768.1	hypothetical protein	-
HHJ45_RS14990	3091974..3092195	-	222	WP_000692298.1	DUF987 domain-containing protein	-
HHJ45_RS14995	3092258..3092734	-	477	WP_001186726.1	RadC family protein	-
HHJ45_RS15000	3092750..3093235	-	486	WP_000214307.1	antirestriction protein	-
HHJ45_RS15005	3093327..3094145	-	819	WP_001234732.1	DUF945 domain-containing protein	-
HHJ45_RS15010	3094245..3094478	-	234	WP_001119717.1	DUF905 family protein	-
HHJ45_RS15015	3094557..3095012	-	456	WP_001504120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T156509 WP_000854686.1 NZ_CP053281:c3090800-3090417 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T156509 NZ_CP069066:c1577751-1577350 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGGCCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT156509 WP_001285602.1 NZ_CP053281:c3091261-3090881 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT156509 NZ_CP069066:c1578005-1577748 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA