Detailed information of TA system TA156498

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1915412..1916243
• Replicon: chromosome
• Accession: NZ_CP053281
• Organism: Escherichia coli strain SCU-308

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: HHJ45_RS09295
• Protein ID: WP_000854814.1
• Coordinates: 1915412..1915786 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: HHJ45_RS09300
• Protein ID: WP_001285585.1
• Coordinates: 1915875..1916243 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ45_RS09255	1910808..1911974	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
HHJ45_RS09260	1912093..1912566	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
HHJ45_RS09265	1912764..1913822	+	1059	WP_029364112.1	FUSC family protein	-
HHJ45_RS09270	1913994..1914323	+	330	WP_000450409.1	DUF496 family protein	-
HHJ45_RS09275	1914424..1914606	-	183	WP_001016348.1	hypothetical protein	-
HHJ45_RS09280	1914678..1914806	+	129	Protein_1813	transposase domain-containing protein	-
HHJ45_RS09285	1915095..1915208	-	114	WP_001161660.1	DUF957 domain-containing protein	-
HHJ45_RS09290	1915221..1915415	-	195	WP_000988600.1	hypothetical protein	-
HHJ45_RS09295	1915412..1915786	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
HHJ45_RS09300	1915875..1916243	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHJ45_RS09305	1916317..1916538	-	222	WP_000692323.1	DUF987 domain-containing protein	-
HHJ45_RS09310	1916601..1917077	-	477	WP_001186773.1	RadC family protein	-
HHJ45_RS09315	1917093..1917572	-	480	WP_000860076.1	antirestriction protein	-
HHJ45_RS09320	1917654..1918475	-	822	WP_001234530.1	DUF945 domain-containing protein	-
HHJ45_RS09325	1918696..1919106	-	411	WP_000846713.1	hypothetical protein	-
HHJ45_RS09330	1919122..1919805	-	684	WP_023147547.1	hypothetical protein	-
HHJ45_RS09335	1919941..1921011	-	1071	WP_000102664.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T156498 WP_000854814.1 NZ_CP053281:c1915786-1915412 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T156498 NZ_CP069066:c751687-751304 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT156498 WP_001285585.1 NZ_CP053281:c1916243-1915875 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT156498 NZ_CP069066:c751937-751782 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60