Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 940352..941006 Replicon chromosome
Accession NZ_CP053281
Organism Escherichia coli strain SCU-308

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HHJ45_RS04645 Protein ID WP_000244772.1
Coordinates 940599..941006 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HHJ45_RS04640 Protein ID WP_000354046.1
Coordinates 940352..940618 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ45_RS04615 935640..936383 + 744 WP_000951964.1 SDR family oxidoreductase -
HHJ45_RS04620 936440..937873 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HHJ45_RS04625 937918..938229 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
HHJ45_RS04630 938393..939052 + 660 WP_000250274.1 hemolysin III family protein -
HHJ45_RS04635 939129..940109 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HHJ45_RS04640 940352..940618 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HHJ45_RS04645 940599..941006 + 408 WP_000244772.1 protein YgfX Toxin
HHJ45_RS04650 941046..941567 - 522 WP_001055874.1 flavodoxin FldB -
HHJ45_RS04655 941679..942575 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HHJ45_RS04660 942600..943310 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HHJ45_RS04665 943316..945049 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T156494 WP_000244772.1 NZ_CP053281:940599-941006 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156494 NZ_CP069066:701278-701679 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156494 WP_000354046.1 NZ_CP053281:940352-940618 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156494 NZ_CP069066:701036-701281 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References