Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3738626..3739167 Replicon chromosome
Accession NZ_CP052877
Organism Escherichia coli strain C21

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag HJD21_RS17980 Protein ID WP_000615982.1
Coordinates 3738889..3739167 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HJD21_RS17975 Protein ID WP_000729704.1
Coordinates 3738626..3738886 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJD21_RS17950 3733662..3734801 + 1140 WP_000785664.1 flagellar type III secretion system protein FlhB -
HJD21_RS17955 3734785..3736878 + 2094 WP_001583165.1 flagellar biosynthesis protein FlhA -
HJD21_RS17960 3736983..3737261 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HJD21_RS17965 3737254..3737610 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HJD21_RS17970 3737667..3738440 - 774 WP_000543899.1 C40 family peptidase -
HJD21_RS17975 3738626..3738886 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HJD21_RS17980 3738889..3739167 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HJD21_RS17985 3739323..3740063 + 741 WP_001225679.1 murein L,D-transpeptidase -
HJD21_RS17990 3740034..3740801 - 768 WP_000333380.1 class II glutamine amidotransferase -
HJD21_RS17995 3741007..3741585 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T155716 WP_000615982.1 NZ_CP052877:3738889-3739167 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T155716 NZ_CP068812:3569710-3570000 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT155716 WP_000729704.1 NZ_CP052877:3738626-3738886 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT155716 NZ_CP068812:3569478-3569720 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References