Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/-
Location 2799650..2800305 Replicon chromosome
Accession NZ_CP052858
Organism Pseudomonas sp. ADAK20

Toxin (Protein)


Gene name higB Uniprot ID A0A1S1UXS8
Locus tag HKK56_RS12350 Protein ID WP_070995472.1
Coordinates 2799650..2799994 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1S1UVI2
Locus tag HKK56_RS12355 Protein ID WP_070995473.1
Coordinates 2799991..2800305 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKK56_RS12340 2796408..2797940 + 1533 WP_029293337.1 NADH-quinone oxidoreductase subunit M -
HKK56_RS12345 2797948..2799411 + 1464 WP_029293335.1 NADH-quinone oxidoreductase subunit NuoN -
HKK56_RS12350 2799650..2799994 + 345 WP_070995472.1 toxin Toxin
HKK56_RS12355 2799991..2800305 + 315 WP_070995473.1 transcriptional regulator Antitoxin
HKK56_RS12360 2800379..2802121 - 1743 WP_029293329.1 ABC transporter substrate-binding protein -
HKK56_RS12365 2802288..2802560 - 273 WP_029293327.1 DUF2160 domain-containing protein -
HKK56_RS12370 2802571..2803371 - 801 WP_017735079.1 carbohydrate ABC transporter permease -
HKK56_RS12375 2803382..2804248 - 867 WP_029293322.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(41-100)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13470.41 Da        Isoelectric Point: 9.9080

>T155646 WP_070995472.1 NZ_CP052858:2799650-2799994 [Pseudomonas sp. ADAK20]
MDALFIELPPFERYRKDYLTDELFHGFQQELMKNPEAGAVMEGTGGLRKLRFVDERRNKGKRGGLRVIYYWWSGGTQFWL
FTLYGKHEQSDLSPQHKKALKQLLDREINARGFT

Download         Length: 345 bp

>T155646 NZ_CP068810:1045521-1045928 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11838.58 Da        Isoelectric Point: 10.5386

>AT155646 WP_070995473.1 NZ_CP052858:2799991-2800305 [Pseudomonas sp. ADAK20]
MKRDIYSELVEGFDALANERQGKVTLRTHTVQQNKLAPLTAEDVIAVRQQLNLSRAVFAMYLRTNTRTLENWEQGRARPN
AQAATLIRLVSKFPDTVERLALLS

Download         Length: 315 bp

>AT155646 NZ_CP068810:1045274-1045540 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S1UXS8

Antitoxin

Source ID Structure
AlphaFold DB A0A1S1UVI2

References